Regulatory

Part:BBa_K3844004:Design

Designed by: Anya Sabhnani   Group: iGEM21_DNHS_SanDiego_CA   (2021-10-21)


NR1D1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
    Illegal NotI site found at 1283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
    Illegal NgoMIV site found at 765
    Illegal NgoMIV site found at 969
    Illegal NgoMIV site found at 1011
    Illegal NgoMIV site found at 1203
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.


Source

DNA Binding Site

References